Tai Te Wu's Analytical Molecular Biology PDF

By Tai Te Wu

ISBN-10: 0306468921

ISBN-13: 9780306468926

ISBN-10: 0792374479

ISBN-13: 9780792374473

Analytical Molecular Biology illustrates the significance of straightforward analytical equipment utilized to a couple uncomplicated molecular biology difficulties, with an emphasis at the significance of organic difficulties, instead of the complexity of arithmetic.
First, the ebook examines an important experimental facts for a selected challenge. Mathematical versions will then be developed with particular inclusion of organic evidence. From such types, predictions will be deduced after which recommend additional experimental reviews. a number of vital molecular biology difficulties should be mentioned within the order of the complexity of the mathematical types. in response to such illustrations, the readers can then enhance their very own analytical ways to learn their very own difficulties.
This publication is for somebody who understands they should follow mathematical versions to biology, yet does not inevitably are looking to, from training researchers seeking to gather extra analytical instruments to complex scholars looking a transparent, explanatory text.

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Additional resources for Analytical Molecular Biology

Sample text

21 In order to maintain the reading frames in the V-gene and the J-minigene, so that the resulting protein can be functional, the total number of nucleotides of the segment coding for CDRH3 must be divisible by three. However, the number of nucleotides of the N/P segments on both ends of the D-minigene can vary. As a result, all three reading frames of any D-minigene can be used, as long as there is no termination codon. Furthermore, since the recombination signal sequences on both sides of the D-minigene to join D to J and V to D are similar, D-minigenes can be inverted.

21 In order to maintain the reading frames in the V-gene and the J-minigene, so that the resulting protein can be functional, the total number of nucleotides of the segment coding for CDRH3 must be divisible by three. However, the number of nucleotides of the N/P segments on both ends of the D-minigene can vary. As a result, all three reading frames of any D-minigene can be used, as long as there is no termination codon. Furthermore, since the recombination signal sequences on both sides of the D-minigene to join D to J and V to D are similar, D-minigenes can be inverted.

Nat. Acad. Sci. USA, 53, 1403-1409. Johnson G and Wu TT (1999) Random length assortment of human and mouse T cell receptor for antigen and chain CDR3. Immunol. , 77, 391 -394. Johnson G and Wu TT (2000) Kabat database and its applications: 30 years after the first variability plot. Nucl. , 28, 214-218. Kabat EA (1976) Structural Concepts in Immunology and Immunochemistry, 2nd Edition. Holt, Rinehart and Winston, New York. Kabat EA, Wu TT and Bilofsky H (1978) Variable region genes for the immunoglobulin framework are assembled from small segments of DNA—A hypothesis.

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Analytical Molecular Biology by Tai Te Wu


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